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ZebraTrack: An Automated Method to Study Zebrafish Behaviour

Name: ZebraTrack: An Automated Method to Study Zebrafish Behaviour
Brand: LAP Lambert Academic Publishing
SKU: 16014008
Price: 30.22 EUR
Availability: InStock
Author: Yogesh Bhargava
ISBN: 9783659900518

Yogesh Bhargava

Anamika Bhargava

Jezik

Angleščina

Knjiga Mehka

Koda Libristo: 16014008

Založba LAP Lambert Academic Publishing, november 2016

Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we... Celoten opis

Koda Libristo: 16014008

73 b

30.22 €

Na zalogi pri dobavitelju Odposlali bomo v 5-8 dneh

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Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we propose a novel method which we called as ZebraTrack. It includes cost-effective imaging setup for distraction-free behavioural acquisition, automated tracking using open-source ImageJ software and workflow for extraction of behavioural endpoints. Our ImageJ algorithm is capable of providing control to users at key steps while maintaining automation in tracking without the need for the installation of external plugins. We have validated this method by testing novelty induced anxiety behaviour in adult zebrafish. Our results, in agreement with established findings, showed that during state-anxiety, zebrafish showed reduced distance travelled, increased thigmotaxis and freezing events. Furthermore, we proposed a method to represent both spatial and temporal distribution of choice-based behaviour which is currently not possible to represent using simple videograms. ZebraTrack method is simple and economical, yet robust enough to give results comparable with those obtained from costly proprietary software like Ethovision XT.