Terpene synthases

Terpene Synthases, Volume 700 in the Methods in Enzymology series, continues the legacy of this highly respected laboratory standard with its first dedicated collection on this important family of enzymes. Terpene synthases are a diverse set of enzymes that use exquisite mechanisms to form complex (poly)cyclic hydrocarbon skeletons. Chapters in this Volume include Structural analysis by X-ray crystallography and cryo-EM, Understanding mechanisms using stable isotopes, substrate analogs, or computational tools, Engineering fusion enzymes, Ancestral terpene cyclases, as well as the Sequence, structure, and function of non-canonical terpene synthases. The only collection of current methodology for the investigation of terpene synthases, with topics including from bioinformatics, enzymology, computational chemistry, and engineeringChapters authored by international experts in the fieldThe latest contribution in the leading serial Methods in Enzymology