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Techniques and Protocols for Evaluating Nanosystems: From Design and Development to Biological Applications is a comprehensive guide on the main techniques used in the characterization of nanoparticulate systems. Covering a wide range of techniques such as thermal analysis, imaging, light scattering, analytical, optical spectroscopic, and statistical and AI-based optimization techniques, it provides thorough explanations of each technique, highlighting their advantages, limitations, and providing characterization protocols and case studies for their application. Special attention is given to the regulatory and pharmaceutical industry requirements. This book offers researchers and advanced students a valuable resource on application of these techniques in the fields of nanotechnology, pharmaceutical technology, and chemical engineering.