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Stem cells
In vitro neurogenesis of human and rat bone marrow derived mesenchymal stem cells
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136 b
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This study aimed to investigate the ability of mesenchymal stem cells derived from bone marrow of human and rat to transdifferentiate into functional neural like cells. A study was made on two different models (human and rat) using two different techniques (direct plating, and density gradient method) respectively. Immunophenotypic analysis demonstrated that human and rat BM-MSCs at passage three uniformly positive for CD44 sub-population. However, MSCs were always found to be negative for heamatopoietic specific markers CD34. To explore the possibility of BM-MSCs in vitro transdifferentiation, the expanded MSCs from third passage were allowed to reach 80% confluence and induced to differentiate into neuron like cells by an enhanced three-step protocol. The identity of the NLCs were illustrated by the analysis of morphology using immunocytochemistry.
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