Separation Science and Proteomics

Separation Science and Proteomics: Current Trends and New Approaches provides readers with advance theory and methodology for the most important separation methods contributing to current proteomics. Intended to be a practical reference that meets the needs of everyday practitioners, it will also serve as a go-to guide for scientists focused on the theory of separation science, new methods development and new equipment design. Future progress in proteomics is connected to the introduction of new and more powerful techniques as well as the perfection of techniques proposed over the past several decades. An in-depth understanding of physical mechanisms for a particular separation method or methods combination is crucial for scientists who need to bridge the gap between traditional and contemporary methods. Authored by a chemist who has more than 20 years of experience in separation science and proteomics, this book offers readers insights and solutions to the separation mechanism of a full range of related techniques complex problems, including the concentration distribution of analysed protein during IEF separation in natural or IPG gradients or detailed mechanisms of electrophoresis in thermal gradients. Covers theoretical aspects such as resolving power limitations as flow rate and electric current density reach technical limits Provides a critical review of newly proposed separation methods and approaches, including a range of equilibrium gradient methodsAddresses the physical aspects important for optimization of the separation process, including acid-base equilibria, dissociation schemes, and chemistry of non-aqueous solutionsAnalyzes biophysical methods of protein structure analysis and discusses scaling to micro-separations and possible applications of nanotechnologies