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All living organisms require nitrogen, and a single§process biological nitrogen fixation accounts for§more than 97% of natural nitrogen inputs into Earth s§ecosystems. To date, most nitrogen fixation research§has focused on the symbiotic nitrogen fixation§occurring via the structured relationship of§particular bacteria and plant roots. Yet free-living§nitrogen fixation, the nitrogen fixation occurring§outside of this symbiosis, may represent the dominant§source of biologically-available nitrogen to a§variety of ecosystems. Here we explored free-living§nitrogen fixation controls, ranging from the effect§of fertilization on nitrogen inputs to the role of§microbial nitrogen-fixer community structure in§regulating nitrogen fixation rates. We found that§free-living nitrogen fixation was a dynamic process§that supplied diverse ecosystems with nitrogen, and§that we could elucidate multiple controls over this§important process.
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