Practical Biochemistry

1. Laboratory Apparatus and Equipment, Good Safe Lab Practice and Waste Management
2. Estimation of pH by pH Meter and ABG Analyzer
3. Qualitative Assessments: Analysis of Normal Urine
4. Analysis of Abnormal Constituents in the Urine and their Clinical Correlation
5. Screening of Urine for Inborn Errors of Metabolism and Paper Chromatography
6. Paper Chromatography
7. Principles of Colorimetry and Spectrophotometry
8. Estimation of Glucose in Serum and Other Biological Fluids
9. Estimation of Urea in Serum and Other Biological Fluids
10. Estimation of Creatinine in Serum and Other Biological Fluids
11. Estimation of Uric Acid in Serum
12. Estimation of Total Protein in Serum and Other Biological Fluids
13. Estimation of Total Cholesterol and HDL Cholesterol
14. Estimation of Triglycerides
15. Estimation of Bilirubin
16. Estimation of Calcium
17. Estimation of Phosphorus
18. Estimation of AST or SGOT and ALT or SGPT
19. Estimation of Alkaline Phosphatase (ALP)
20. Cerebrospinal Fluid Analysis
21. Electrophoresis of Serum Proteins
22. Estimation of Serum Electrolytes by lon Selective Electrodes
23. Enzyme-linked Immunosorbent Assay (ELISA)
24. Immunodiffusion
25. DNA Isolation and Quantification
26. Autoanalyzers
27. Quality Control in Laboratory
28. Biochemical Tests in Diabetes Mellitus
29. Interpretation of Laboratory Result: Carbohydrate Metabolism
30. Interpretation of Laboratory Result: Oral Glucose Tolerance Test
31. Biochemical Tests in Gout, Thyroid Disorders, Pancreatitis, Acid Base Disorders and Edema
32. Biochemical Tests in Nephrotic Syndrome, Proteinuria, Renal Failure, Dyslipidemia and Myocardial Infarction
33. Liver Function Test: An Overview
34. Kidney Function Test: An Overview
35. Pancreatic and Gastric Function Tests
36. Enzymes as Disease Markers
37. Interpretation of Laboratory Result: Amino Acid Metabolism
38. Interpretation of Laboratory Result: Protein Metabolism
39. Interpretation of Laboratory Result: Lipid Metabolism
40. Interpretation of Laboratory Result: Purine Nucleotide Metabolism
41. Clinical Manifestations of Deficiencies Associated with Vitamins and Minerals
42. Energy Content of Food Items and Glycemic Index.
43. Baseline Diagnostic Prognostic and Discharge Investigations of Clinical Biochemistry
44. Point-of-Care Testing/Bedside Testing

Appendix
Index