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This book provides the current status of paper-based diagnostic solutions, or Microfluidics 2.0. This book explores how paper-based tests can be widely distributed and utilized by semi-skilled personnel, how close to commercial applications the technology has become, and what is still required to make paper-based diagnostics the game-changer it can be. The technology is examined through the lens of the World Health Organization's ASSURED criteria for low-resource countries (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free, and Deliverable to end-users), and the applications it has to health technology, environmental technology, food safety, and more. It is appropriate for researchers in these areas, as well as those interested in microfluidics, and has chapters dedicated to principles such as Theory of Flow and surface treatments; components such as biomarkers and detection; and current methods of manufacturing.