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Microinjection and Transgenesis
Strategies and Protocols
Angleščina
115 b
Do 30 dni za vračilo
The technique of microinjection is routinely used to transfer biological materials - proteins, oligonucleotides, RNA and DNA - into cultured somatic cells and embryos. This manual provides over 100 protocols for microinjection and useful auxillary techniques, as well as the most relevant strategies that make this field a highly productive area of biological and biotechnological research. Special topics include: the use of YACs to produce transgenic mice, ligand-dependent site-specific recombination in embryonic stem cells, morula aggregation to generate germline chimeras as well as the analysis of injected cells.
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