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HPTLC METHOD
Angleščina
124 b
30 dni za vračilo blaga
A stability-indicating high-performance thin-layer chromatography (HPTLC) method was developed and validated for determination of Vibegron. Optimization of conditions for the spectro densitometric procedure was reached by eluting HPTLC pre-coated silica gel aluminium plates in horizontal chamber. The solvent system consisted of Acetone. This system was found to give compact, dense and typical peak for Vibegron. Densitometric analysis of the drug was carried out in the reflectance mode at 254 nm by using a computer controlled densitometric scanner. The calibration curve Vibegron was linear in the range of 200-1000 ng/band. The method was validated for precision, robustness and recovery. Vibegron was subjected for stability study at acid, base, oxidation, thermal and photo- degradation condition, among which thermal degradation had the highest degradative.
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