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Fast and Cheap Protein Purification
Angleščina
142 b
Do 30 dni za vračilo
Two naturally produced in vivo affinity resin §substitutes have been combined with a self-cleaving §tag to develop two novel affinity-based purification §technologies. The first resin alternative is §polyhydroxybutyrate (PHB) granules and the second is §elastin-like polypeptides (ELP). An engineered §tripartite protein fusion is expressed in E. coli §and purified through simple centrifugation. The §intein self-cleaving tag then releases the target §protein with a mild pH shift. §§The two systems have been successfully used at §laboratory scale to purify more than a dozen §proteins varying in size, complexity and activity, §demonstrating the proof of principle, high purity §and yield attainable. Hence, the cost associated §with purification of recombinant proteins is reduced §significantly to effectively that of just the §culture medium. It is expected that this §combination of improved economics and simplicity §will constitute a significant breakthrough in both §large-scale production of purified proteins and §enzymes as well as high-throughput proteomics §studies.
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