Circadian Rhythms

Circadian Rhythms: Methods and Protocols is an in-depth examination ofcircadian biology, presented by leading researchers in the field. Thiscutting-edge collection of protocols is adaptable for research at everylevel, and represents the huge strides that chronobiologists have madeover the past two decades. These robust, reproducible procedures takeinto account the myriad of variables that circadian researchers must becognizant of while conducting their research. By distilling thearsenal of methodologies available, and presenting essential proceduresin precise detail, Circadian Rhythms: Methods and Protocols supports avast majority of circadian research efforts. Part I of this seven-part volume provides a concise introduction toessential components of circadian biology, including light,photoreceptors, circadian clocks, and statistical analysis. Part IIpresents protocols and techniques for monitoring and analyzing rhythmicpatterns of several model organisms. Parts III, IV, and V emphasizethe identification of clock genes and protein and RNA expression incircadian biology. Protocols for in vitro systems comprise Part VI,and Part VII provides an outline of methods and tips for microscopyanalysis. Circadian biologists at all research levels will realizetremendous benefit from this extraordinary collection of methods andprotocols for the study of circadian rhythms.